Chemistry and Biochemistry
Dr. Lauren Toote
Testing for Lyme disease has proven to be time consuming and inaccurate, especially in its early stages. The goal of this project is to develop a new diagnostic that has improved timeliness, sensitivity, and selectivity. This test is based on an enzyme-linked immunosorbent assay, or ELISA. To improve on what is currently in place, magnetic beads will be used. Unlike a normal ELISA, these magnetic beads are the binding site of the capture antibody instead of the microplate surface. With greatly increased surface area from the beads, the process can be much faster and detect far lesser concentrations of protein with greater accuracy. This test will allow for more reliable diagnosis, reducing progression of the disease and chronic affects. Through this research a magnetic bead assay for detection of the human response to Lyme disease was successfully developed and results were achieved in 90 minutes.
Cosgrove, Justin, "Development of Sensitive and Fast Immunoassay for Lyme Disease" (2022). Summer Scholarship, Creative Arts and Research Projects (SCARP). 18.
Scholarship, Creative Arts, and Research Project (SCARP)