Title
Optimization of the Control Line on a Lateral Flow Assay for Lead (II) Detection in Drinking Water
Document Type
Poster
Date
Summer 2022
Academic Department
Chemistry and Biochemistry
Faculty Advisor(s)
Dr. Lauren Toote
Abstract
According to a recent survey by the United States Environmental Protection Agency (EPA), there are between 6 to 10 million lead (Pb2+) service lines in the country. Consumption of lead can cause brain and kidney damage. Our goal is to create a simple, inexpensive paper-based assay to make lead testing accessible. Lateral flow assays (LFAs) are rapid tests (5-30 mins) used to detect or quantify analytes in mixtures. Each LFA has a control line and a test line(s). The control line ensures the test is working. In our test, the control line is composed of neutral red polymer nanoparticles, which are pH sensitive, resulting in a colorimetric change when a water sample is added. Nanoparticles with a size of 180 nm were synthesized and analyzed using UV-Vis and DLS. The control line signal on the lateral flow assay was best five minutes after tap water was added.
Recommended Citation
Sanchez, Yudeliz, "Optimization of the Control Line on a Lateral Flow Assay for Lead (II) Detection in Drinking Water" (2022). Summer Scholarship, Creative Arts and Research Projects (SCARP). 23.
https://jayscholar.etown.edu/scarp/23
Notes
Scholarship, Creative Arts, and Research Project (SCARP)