Optimization of the Control Line on a Lateral Flow Assay for Lead (II) Detection in Drinking Water
Chemistry and Biochemistry
Dr. Lauren Toote
According to a recent survey by the United States Environmental Protection Agency (EPA), there are between 6 to 10 million lead (Pb2+) service lines in the country. Consumption of lead can cause brain and kidney damage. Our goal is to create a simple, inexpensive paper-based assay to make lead testing accessible. Lateral flow assays (LFAs) are rapid tests (5-30 mins) used to detect or quantify analytes in mixtures. Each LFA has a control line and a test line(s). The control line ensures the test is working. In our test, the control line is composed of neutral red polymer nanoparticles, which are pH sensitive, resulting in a colorimetric change when a water sample is added. Nanoparticles with a size of 180 nm were synthesized and analyzed using UV-Vis and DLS. The control line signal on the lateral flow assay was best five minutes after tap water was added.
Sanchez, Yudeliz, "Optimization of the Control Line on a Lateral Flow Assay for Lead (II) Detection in Drinking Water" (2022). Summer Scholarship, Creative Arts and Research Projects (SCARP). 23.
Scholarship, Creative Arts, and Research Project (SCARP)