Title

Immunological Properties of Lactate Dehydrogenase Isozymes in Trout

Document Type

Article

Publication Title

Transactions of the American Fisheries Society

Publication Date

1-1-1978

Abstract

Evolutionary and immunological relationships among the different genes specifying synthesis of rainbow trout (Salmo gairdneri) LDH subunits were demonstrated. A procedure was devised for the purification and concentration of specific rainbow trout isozymes which were then used for antisera production. The B41 and B42 autotetramer isozymes and the Group “a” isozyme system were shown by double agar diffusion to produce distinctly different antibodies. Antisera against specific isozymes showed degrees of cross-reaction depending on the relatedness of the isozymes. The LDH-C gene locus is more closely related to the LDH-B2 gene than to the LDH-B1 gene. Electrophoresis was demonstrated to be an efficient additional method to spectrophotometric assays to show inactivation of LDH by antibodies. LDH isozymes underwent rapid conformational change when purified and stored. © 1978 by the American Fisheries Society.

Volume

107

Issue

2

First Page

295

Last Page

304

DOI

10.1577/1548-8659(1978)107[295:IPOLDI]2.0.CO;2

ISSN

00028487

E-ISSN

15488659

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