Title
Combined infusion and stimulation with fast-scan cyclic voltammetry (CIS-FSCV) to assess ventral tegmental area receptor regulation of phasic dopamine
Document Type
Article
Publication Title
Journal of Visualized Experiments
Publication Date
4-1-2020
Abstract
Phasic dopamine (DA) release from the ventral tegmental area (VTA) to the nucleus accumbens plays a pivotal role in reward processing and reinforcement learning. Understanding how the diverse neuronal inputs into the VTA control phasic DA release can provide a better picture of the circuitry that controls reward processing and reinforcement learning. Here, we describe a method that combines intra-VTA cannula infusions of pharmacological agonists and antagonists with stimulation-evoked phasic DA release (combined infusion and stimulation, or CIS) as measured by in vivo fast-scan cyclic voltammetry (FSCV). Using CIS-FSCV in anesthetized rats, a phasic DA response can be evoked by electrically stimulating the VTA with a bipolar electrode fitted with a cannula while recording in the nucleus accumbens core. Pharmacological agonists or antagonists can be infused directly at the stimulation site to investigate specific VTA receptors' roles in driving phasic DA release. A major benefit of CIS-FSCV is that VTA receptor function can be studied in vivo, building on in vitro studies.
Volume
2020
Issue
158
DOI
10.3791/60886
ISSN
1940087X
PubMed ID
32420985
Recommended Citation
Wickham, Robert J.; Lehr, Makenzie; Mitchell, Lauryn; and Addy, Nii A., "Combined infusion and stimulation with fast-scan cyclic voltammetry (CIS-FSCV) to assess ventral tegmental area receptor regulation of phasic dopamine" (2020). Faculty Publications. 866.
https://jayscholar.etown.edu/facpubharvest/866
