Title
Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA
Document Type
Book Chapter
Publication Title
Methods in Molecular Biology
Publication Date
1-1-2020
Abstract
Because of the important roles noncoding RNAs play in gene expression, their sequence-specific recognition is important for both fundamental science and the pharmaceutical industry. However, most noncoding RNAs fold in complex helical structures that are challenging problems for molecular recognition. Herein, we describe a method for sequence-specific recognition of double-stranded RNA using peptide nucleic acids (PNAs) that form triple helices in the major grove of RNA under physiologically relevant conditions. We also outline methods for solid-phase conjugation of PNA with cell-penetrating peptides and fluorescent dyes. Protocols for PNA preparation and binding studies using isothermal titration calorimetry are described in detail.
Volume
2105
First Page
157
Last Page
172
DOI
10.1007/978-1-0716-0243-0_9
ISSN
10643745
E-ISSN
19406029
PubMed ID
32088869
Recommended Citation
Brodyagin, Nikita; Hnedzko, Dziyana; MacKay, James A.; and Rozners, Eriks, "Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA" (2020). Faculty Publications. 877.
https://jayscholar.etown.edu/facpubharvest/877
