Histone H2A Repression Domain Regulates Cell-Cycle Progression in Saccaromyces Cerevisea

Presentations Session 2 - Hoover 212

This study researched the histone H2A's N-terminal tail amino acid sequence, focusing on amino acids 16-20 known as the histone H2A repression, HAR, domain. This study found a sensitivity within the deletion of the HAR domain to hydroxyurea, a genotoxin known to show defects in DNA replication. A spotting assay concluded that the HAR domain has an important regulator of the DNA replication process. The RNR1-4 genes were studied through reverse transcriptase polymerase chain reactions in order to see the difference in wild type gene expression compared to the deletion of the HAR domain. The deletion of the HAR domain showed a repression of the RNR2 gene which could be a reason why the DNA replication machinery does not work within the mutant strains. This study shows that the HAR domain has an important regulation of the process DNA replication possibly due to the RNR2 gene and the RNR protein complex.