Document Type

Student Research Paper

Date

Summer 2022

Academic Department

Biology

Faculty Advisor(s)

Dr. Jane Cavender

Abstract

SV40 T-ag is capable of transforming rodent cells in culture and inducing tumor formation in animal models through interactions with cellular proteins. The TEAD-1/TEF-1 transcription factor, discovered complexed with T-ag, activates early viral gene expression and is essential for the YAP/TAZ proliferation and anti-apoptosis pathway. Studies in rat cells showed expression of T-ag induced an alternatively spliced isoform of TEAD1; thus, this study investigated if this isoform existed in human cells and what role it may play in transformation. Using T-ag-transformed HDFs, TEAD1 was assessed by RT-PCR and immunoblot. Preliminary RT-PCR data showed 2 of 3 primer sets exhibited a banding pattern shorter than predicted. Of the several expected isoforms, only the 37 kDa was detected by immunoblotting in all cell lines. Co-immunoprecipitation studies are underway to determine if the 37 kDa isoform is complexed with T-ag, and additional PCR primer sets will determine if C-terminal splice variants exist.

Notes

Scholarship, Creative Arts, and Research Project (SCARP)

Included in

Biology Commons

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.